International archives of allergy and immunology
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Int. Arch. Allergy Immunol. · Jul 2000
A sensitive monoclonal antibody sandwich ELISA for the measurement of the major olive pollen allergen Ole e 1.
Olive pollen is a major cause of inhalant allergy in countries around the Mediterranean sea. The major allergen of olive pollen is Ole e 1. Measurement of the major allergen content of allergen products for diagnosis and therapy is becoming an essential element of standardization protocols. This study aimed at the development of a monoclonal antibody (mAb) sandwich ELISA for Ole e 1. ⋯ Two sensitive sandwich ELISAs for the major olive pollen allergen Ole e 1 were developed. They will prove to be useful tools in allergen standardization protocols.