Shock : molecular, cellular, and systemic pathobiological aspects and therapeutic approaches : the official journal the Shock Society, the European Shock Society, the Brazilian Shock Society, the International Federation of Shock Societies
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Biography Historical Article
Shock at the millennium II. Walter B. Cannon and Lawrence J. Henderson.
Walter B. Cannon and Lawrence J. Henderson, students of shock in the early twentieth century, were contemporaries for four decades in the Harvard Department of Physiology. ⋯ In delineation, Cannon described mechanisms; Henderson described the organization of systems. Cannon's emphasis on homeostasis with the identification of feedback arcs dominated shock research for the balance of the twentieth century. Henderson's perspective designating the importance of organization to those restorative mechanisms could well reemerge to dominate the twenty-first.
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Previously, we have documented that gut-derived lymph from rats subjected to trauma/hemorrhagic shock (T/HS) is injurious to human umbilical vein endothelial cells (HUVEC). To verify these findings in an all rat systems, the ability of T/HS lymph to increase rat pulmonary microvascular endothelial cell (RPMVEC) monolayer permeability and kill RPMVEC was compared with that observed with HUVECs. RPMVEcs isolated from male rats or HUVECs were grown in 24-well plates for the cytotoxicity assays or on permeable filters in a two-chamber system for permeability assays. ⋯ Similarly, incubation with 10% T/HS lymph increased the permeability of both HUVEC and RPMVEC monolayers more than 2-fold, even with an incubation period as short as 1 h. In conclusion, these results provide further evidence that T/HS lymph, but not T/SS lymph or post-T/HS portal vein plasma, is injurious to endothelial cells and that RPMVECs are as susceptible to injury as HUVECs. Additionally, these studies support the emerging concept that gut-induced distant organ injury is mediated by factors contained in mesenteric lymph.