Microcirculation : the official journal of the Microcirculatory Society, Inc
-
In sepsis, tumor necrosis factor-alpha (TNF-alpha) contributes to endothelial barrier breakdown. The involvement of Rho A/rho kinase signaling has recently been challenged. Here, we tested the role of cAMP and Rac 1 signaling. ⋯ Our data indicate that decrease of cAMP and Rac 1 inactivation, rather than Rho A activation, contribute to TNF-alpha-induced endothelial barrier breakdown in vivo and in vitro.