Biochemical and biophysical research communications
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Biochem. Biophys. Res. Commun. · May 2010
Spinal high-mobility group box 1 contributes to mechanical allodynia in a rat model of bone cancer pain.
Mechanisms underlying bone cancer-induced pain are largely unknown. Previous studies indicate that neuroinflammation in the spinal dorsal horn is especially involved. Being first reported as a nonhistone chromosomal protein, high-mobility group box 1 (HMGB1) is now implicated as a mediator of inflammation. ⋯ Tumor cells inoculation significantly increased HMGB1 expression in the spinal dorsal horn, while intrathecal injecting a neutralizing antibody against HMGB1 showed an effective and reliable anti-allodynia effect with a dose-dependent manner. IL-1beta was significantly increased in cancer pain rats while intrathecally administration of anti-HMGB1 could decrease IL-1beta. Together with previous reports, we predict that bone cancer induces HMGB1 production, enhancing spinal IL-1beta expression and thus modulating spinal excitatory synaptic transmission and pain response.