Aviat Space Envir Md
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Aviat Space Envir Md · Aug 2014
Optimal 3D culture of primary articular chondrocytes for use in the rotating wall vessel bioreactor.
Reliable culturing methods for primary articular chondrocytes are essential to study the effects of loading and unloading on joint tissue at the cellular level. Due to the limited proliferation capacity of primary chondrocytes and their tendency to dedifferentiate in conventional culture conditions, long-term culturing conditions of primary chondrocytes can be challenging. The goal of this study was to develop a suspension culturing technique that not only would retain the cellular morphology, but also maintain the gene expression characteristics of primary articular chondrocytes. ⋯ Our results provide a novel and alternative culturing technique for primary chondrocytes suitable for studies that require suspension such as those using the rotating wall vessel bioreactor. In addition, we provide an alternative culturing technique for primary chondrocytes that can impact future mechanistic studies of osteoarthritis progression, treatments for cartilage damage and repair, and cartilage tissue engineering.
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Aviat Space Envir Md · Jul 2014
Pilots' visual scan patterns and situation awareness in flight operations.
Situation awareness (SA) is considered an essential prerequisite for safe flying. If the impact of visual scanning patterns on a pilot's situation awareness could be identified in flight operations, then eye-tracking tools could be integrated with flight simulators to improve training efficiency. ⋯ Eye-tracking devices can aid in capturing pilots' visual scan patterns and SA performance, unlike traditional flight simulators. Therefore, integrating eye-tracking devices into the simulator may be a useful method for promoting SA training in flight operations, and can provide in-depth understanding of the mechanism of visual scan patterns and information processing to improve training effectiveness in aviation.
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Aviat Space Envir Md · Jul 2014
Three methods of manual external chest compressions during microgravity simulation.
Cardiopulmonary resuscitation (CPR) in microgravity is challenging. There are three single-person CPR techniques that can be performed in microgravity: the Evetts-Russomano (ER), Handstand (HS), and Reverse Bear Hug (RBH). All three methods have been evaluated in parabolic flights, but only the ER method has been shown to be effective in prolonged microgravity simulation. All three methods of CPR have yet to be evaluated using the current 2010 guidelines. ⋯ All three methods have merit in performing ECC in simulated microgravity; the ER and RBH have adequate ECC rates, and the HS method has adequate D(T). However, all methods failed to meet all criteria for the 2010 guidelines. Further research to evaluate the most effective method of CPR in microgravity is needed.
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Aviat Space Envir Md · Jul 2014
Helicopter pilot scan techniques during low-altitude high-speed flight.
This study examined pilots' visual scan patterns during a simulated high-speed, low-level flight and how their scan rates related to flight performance. As helicopters become faster and more agile, pilots are expected to navigate at low altitudes while traveling at high speeds. A pilot's ability to interpret information from a combination of visual sources determines not only mission success, but also aircraft and crew survival. ⋯ Results may aid training effectiveness specific to helicopter aviation, particularly in high-speed low-level flight conditions.
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The fatality rate for general aviation, 82 times that of air carriers and even higher for those involving aeromedical transportation, has not declined over time. Although noncommercial (private) pilots are increasingly seeking the instrument rating (IFR), which offers safety advantages, there is a paucity of research on causes of fatal crashes for this aviator subset. ⋯ For the IFR-rated private pilot, our study argues for an emphasis on training and currency in SD/FMC and aerodynamic stall recovery. Likewise training to remedy IAD, the frequent cause of fatal night accidents, and flying by instrument flight rules to assure obstacle/terrain clearance should be encouraged for pilots undertaking such operations.