Clin Lab
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Intraosseous (IO) access is a valuable tool in prehospital locations and in emergency departments when other forms of vascular access are unavailable. Creatinine is often used for dose adjustment of drugs that may be administered through intraosseous cannulae. We aimed to study the possibility of analysing creatinine in intraosseous samples and study the accuracy and precision of such measurements. ⋯ Based on our findings intraosseous samples can be used for creatinine determination in emergency settings.
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In patients undergoing cardiac surgery, perioperative coagulopathy and the use of allogenic blood products are independently associated with increased mortality and major perioperative cardiac and non-cardiac adverse events. Hemotherapy should be based on specific hemotherapy algorithms rather than "clinical judgments". However, whether hemotherapy should be based on "classical" conventional laboratory coagulation analyses or Point-of-Care (POC) measures is discussed controversially. ⋯ Unfortunately, the studied hemotherapy--algorithms are very complex and thus hard to integrate into daily practice. In close cooperation of three German University Hospitals, the authors developed and implemented two more comprehensive and practical hemotherapy algorithms that are based on either POC measures or conventional coagulation testing. Here we present and discuss the structure and limitations of these algorithms.
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International guidelines stipulate that primarily cardiac troponin (cTn) assays with a coefficient of variation (CV) < or = 10% at the 99th percentile cutoff should be used for diagnosing myocardial infarction. Point-of-care (POC) assays usually do not meet these criteria. Here, we sought to confirm the manufacturer-recommended 99th percentile cutoff and CV of the POC assay AQT90 FLEX cTnI. ⋯ We confirmed the manufacturer recommended 99th percentile cutoff of 23 ng/L and established a CV of 6.7% at 20 ng/L. These results demonstrated that the POC assay AQT90 FLEX cTnI must be classified as "guideline acceptable".
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The type and frequency of E-cadherin (CDH1) germline variants in China for the early-onset diffuse gastric cancer (EODGC) has not been well established. Our study tend to screen and characterize germline variants for CDH1 gene in EODGC patients and in general population in China. ⋯ No deletions or insertions were found in the CDH1 exon boundaries. All of the variants resulted com- mon polymorphisms. CDH1 germline variants are present in EODGC patients in Chinese population, but they are mainly missense variants with unknown function which are likely associated with lymph node metastasis and OS.