Chinese Med J Peking
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Chinese Med J Peking · Oct 2001
Genotyping and preparation of the recombinant nucleocapsid protein antigen of hantavirus.
To identify new recombinant antigens with potential for diagnosis of hemorrhagic fever with renal syndrome (HFRS) and establish reverse transcription-polymerase chain reaction-restriction fragments length polymorphism (RT-PCR-RFLP) for genotyping of hantavirus. ⋯ The good working titrer of expressed recombinant antigen showed that it has the potential to replace the natural antigen for detecting hantavirus antibodies. On comparison with cELISA, the detection rates for these two methods were 100% and 84.6%, and the coincidence rate was 84.6%. The former had a 15.4% higher sensitivity than the latter. The typing efficiency of RT-PCR-RFLP and sero-typing method was 85% (17/20) and 55% (11/20), respectively, showing that the former was 30% higher than the latter, while their results were highly consistent.
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Chinese Med J Peking · Oct 2001
Immune privilege induced by cotransplantation of islet and allogeneic testicular cells.
To induce islet allograft long-term survival through cotransplantation of islet cells with sertoli cells. ⋯ Cotransplantation of islets with FasL+ sertoli cells induces local immune privilege and allows long-term graft survival without systemic immunosuppression.