J Formos Med Assoc
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One hundred and twenty-four gingival crevicular fluid (GCF) samples were harvested from 13 healthy and 111 diseased gingival sites in 10 patients with periodontitis. The total amount and concentration of interleukin-1 beta (IL-1 beta) in each sample was measured by the enzyme-linked immunosorbent assay (ELISA) technique using an ELISA Kit specific for this cytokine. The IL-1 beta was undetectable in 23% of the GCF from clinically non-inflamed sites (three out of 13), while it could mostly be identified in diseased sites (109/111). ⋯ However, no significant difference of crevicular IL-1 beta concentration (pg/microL) could be found among groups with different clinical parameters. Our results indicate that IL-1 beta was present in the GCF of most clinically non-inflamed gingiva and almost all diseased pockets. The significant elevation of total IL-1 beta amount and GCF volume in diseased sulci suggests that it is closely associated with the severity of periodontal disease.