Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
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Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi · Jul 2010
[Differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells transfected by SOX9 eukaryotic expression vector in vitro].
The biological treatment of intervertebral disc degeneration becomes a research hotspot in recent years. It is necessary to find an effective approach to induce bone marrow mesenchymal stem cells (BMSCs) differentiate to disc cells which could make application of cell transplantation as a treatment of intervertebral disc degeneration. To investigate the effects of the recombinant plasmid pcDNA3.1IE-SOX9Flag on differentiation of rabbit BMSCs into nucleus pulposus-like cells. ⋯ The recombinant plasmid pcDNA3.1IE-SOX9Flag can be successfully transfected into rabbit BMSCs, the transfected BMSCs can differentiate into nucleus pulposus-like cells, which lays a theoretical foundation for treatment of intervertebral disc degeneration with BMSCs transplantation.