Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
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Zhongguo Yi Xue Ke Xue Yuan Xue Bao · Apr 2021
Review[Research Progress on Role and Mechanism of Elabela in Organ Fibrosis].
Elabela is a newly discovered peptide in recent years. It is the endogenous ligand of Apelin receptor(APJ)and plays an important role in embryonic development and adult organs. Elabela-APJ axis is closely related to organ fibrosis. ⋯ In addition,it can prevent kidney and heart fibrosis by down-regulating the expression of fibrosis and inflammatory factors. However,there is a positive correlation between the level of Elabela and the degree of liver fibrosis,suggesting that Elabela may play a role in promoting liver fibrosis. This review aims to explore the role of Elabela-APJ axis in renal fibrosis,cardiac fibrosis,and liver fibrosis,and to provide a new therapeutic target for organ fibrosis.
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Zhongguo Yi Xue Ke Xue Yuan Xue Bao · Apr 2021
[Dexmedetomidine-mediated Wnt Pathway Inhibits Sevoflurane-induced Cognitive Impairment in Neonatal Rats].
Objective To explore the effect of dexmedetomidine(Dex)on sevoflurane-induced cognitive impairment in neonatal rats through Wnt signaling pathway. Methods Sixty 7-day-old SD rats were assigned into five groups:control group(without any intervention),Dex group(intraperitoneal injection of 25 μg/kg Dex),sevoflurane group(3% sevoflurane treatment for 4 hours),sevoflurane+Dex group(inhalation of 3% sevoflurane after injection of 25 μg/kg Dex for 4 hours),and sevoflurane+Dex+Wnt inhibitor group(Wnt inhibitor XAV393 and 25 μg/kg Dex were injected and 3% sevoflurane was inhaled for 4 hours). Three weeks later,Morris water maze was used to detect the cognitive function;TdT-mediated dUTP nick end labeling(TUNEL)staining was performed to detect the apoptosis of hippocampal neurons;neuronal nuclei (NeuN) staining was conducted to detect the survival of hippocampal neurons;Western blot was carried out to detect the expression of apoptosis-related proteins. ⋯ Compared with that in the sevoflurane group,the expression of P(ser9)-GSK-3β/GSK-3β were up-regulated in sevoflurane+Dex group(t=8.310,P <0.001). Compared with that in sevoflurane+Dex group,the expression of P(ser9)-GSK-3β/GSK-3β in sevoflurane+Dex+Wnt inhibitor group was down-regulated(t=5.510,P=0.005). Conclusion Dex can mediate Wnt/GSK-3β/β-catenin signaling pathway to inhibit sevoflurane-induced cognitive impairment in neonatal rats.