Revista médica de Chile
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Revista médica de Chile · Mar 1990
Case Reports[Acute post-partum infarct of the myocardium secondary to a spontaneous dissection of the coronary artery].
A 38 year-old woman had an anterolateral wall myocardial infarction and acute left ventricular failure 11 days after a cesarean section. Coronary angiography performed 14 days later because of recurrent angina disclosed dissection of the left main coronary artery. She subsequently underwent aorto coronary bypass surgery uneventfully. Spontaneous coronary artery dissection is a rare entity that mainly affects women in peripartum periods and presents a high mortality rate.
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Revista médica de Chile · Mar 1990
Case Reports[Use of a specific DNA probe to confirm sickle cell anemia in a caucasian woman].
A specific DNA probe containing part of the structural B-globulin gene was used to confirm the diagnosis of sickle cell anemia in a caucasian woman. The patient's genomic DNA was digested with the restriction endonuclease Dde I, fractioned by agarose electrophoresis and Southern blotting. Molecular hybridization was performed with the DNA probe prepared by chemical labelling with photobiotin. ⋯ Upon digestion with Dde I, the DNA of an individual with the normal genotype containing the enzyme recognition sequence at the site of sickle cell mutation, resulted in 2 fragments of 201 and 175 bp. The pedigree of the patient's caucasian family was studied by Hb electrophoresis. Four out of 7 brothers carried the sickle cell trait.