Zhonghua fu chan ke za zhi
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Zhonghua Fu Chan Ke Za Zhi · Nov 2012
[Clinical application of noninvasive prenatal diagnosis using cell free fetal DNA in maternal plasma].
To investigate the clinical value of non-invasive prenatal diagnosis using cell free fetal DNA (cff-DNA) in maternal blood. ⋯ (1) Cff-DNA in maternal blood can be used to determine fetal gender in early prenancy with considerable sensitivity and specificity. But the trace cff-DNA and the high maternal DNA background might have impact on the result. (2) Analysis of cff-DNA in maternal blood of the second trimester women showed that MPSS could be used for prenatal screening of trisomy 21 and trisomy 18. However, further research should be done for other chromosomes aneuploidy detection.
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Zhonghua Fu Chan Ke Za Zhi · Nov 2012
[Value of detection of cell-free fetal DNA in maternal plasma in the prenatal diagnosis of chromosomal abnormalities].
To investigate the value of detection of fetal cell-free fetal DNA (cff-DNA) in maternal plasma in the prenatal diagnosis of chromosomal abnormalities. ⋯ (1) The non-invasive fetal trisomy test (NIFTY) by next generation sequencing is a safe, accurate and high throughput method for the prenatal diagnosis of trisomy-21. (2) Use NIFTY as a further screening for pregnant women with high-risk serological screening results could lower invasive prenatal diagnosis rate. (3) Cases with positive NIFTY test results should receive amniocentesis and karyotype analysis to confirm the diagnosis before abortion.
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Zhonghua Fu Chan Ke Za Zhi · Nov 2012
[Effect of estrogen on the expression of matrix GLA protein in ovariectomized SD rats].
To investigate the effect of estrogen on expression of matrix GLA protein (MGP) in ovariectomized Sprague-Dawley (SD) rats and the role of estrogen in improving postmenopausal osteoporosis. ⋯ Estrogen could increase the expression of MGP mRNA and protein in ovariectomized rats and might play an important role in improving postmenopausal osteoporosis.
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Zhonghua Fu Chan Ke Za Zhi · Nov 2012
[MicroRNA-16 regulates the proliferation, invasion and apoptosis of ovarian epithelial carcinoma cells in vitro].
To study the role and mechanism of microRNA-16 (miR-16) in the proliferation, invasion and apoptosis of ovarian epithelial carcinoma cells in vitro. ⋯ miR-16 might inhibit the proliferation, invasion of ovarian epithelial carcinoma cells and enhance their sensitivity to apoptotic stimuli via downregulation of the expression of VEGF, MMP-2 and bcl-2 protein.