The Journal of comparative neurology
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Comparative Study
Morphologic analysis and classification of ganglion cells of the chick retina by intracellular injection of Lucifer Yellow and retrograde labeling with DiI.
Retinal ganglion cells (RGCs) of chicks were labeled by using the techniques of intracellular filling with Lucifer Yellow and retrograde axonal labeling with carbocyanine dye (DiI). Labeled RGCs were morphologically analyzed and classified into four major groups: Group I cells (57.1%) with a small somal area (77.5 microm(2) on average) and narrow dendritic field (17,160 microm(2) on average), Group II cells (28%) with a middle-sized somal area (186 microm(2)) and middle-sized dendritic field (48,800 microm(2)), Group III cells (9.9%) with a middle-sized somal area (203 microm(2)) and wide dendritic field (114,000 microm(2)), and Group IV cells (5%) with a large somal area (399 microm(2)) and wide dendritic field (117,000 microm(2)). Of the four groups, Groups I and II were further subdivided into two types, simple and complex, on the basis of dendritic arborization: Groups Is, Ic, and Groups IIs, IIc. ⋯ The branching density of Group I cells was extremely high in the central zone. The chick inner plexiform layer was divided into eight sublayers by dendritic strata of RGCs and 26 stratification patterns were discriminated. The central and peripheral retinal zones were characterized by branching density of dendrites and composition of RGC groups, respectively.
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Comparative Study
Cellular correlates of progressive hearing loss in 129S6/SvEv mice.
Several strains of mice hear well initially but show progressive sensorineural hearing loss. Affected cochlear cell types include all those known to be affected in human age-related hearing loss (ARHL), or presbycusis. Thus these mice have been offered as models of human ARHL. ⋯ Although neuronal loss was not consistently found, two mice showed loss of afferent dendrites and cell bodies in the cochlear apex without inner hair cell loss. Despite multifaceted degeneration, hearing loss in 129S6 mice appears to be best explained by degenerative changes in outer hair cells and in the organ of Corti, conforming to human sensory ARHL. Age-related changes in the apical spiral limbus may promote pathology of the medial organ of Corti and eventual loss of afferent neurons, with possible implications for human neural ARHL.
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We examined the changes of two transcription factors, CREB and c-Jun, in dorsal root ganglia (DRG) after acute (8 or 48 hours) or chronic (10 days) cyclophosphamide (CYP)-induced cystitis. Results showed an increase in the number of p-CREB-immunoreactive (-IR) cells in the L1 and L2 DRG (5-7-fold; P < or = 0.05) as well as L6 and S1 DRG (2-4-fold; P < or = 0.05) after acute and chronic cystitis. The number of p-CREB-IR cells in the L4-L5 DRG was not altered with cystitis. ⋯ Resiniferatoxin reduced CYP-induced up-regulation of p-CREB in DRG, suggesting that cystitis can reveal an altered CREB phosphorylation that may be mediated by capsaicin-sensitive bladder afferents. Colocalization of p-CREB and Trk receptor(s) showed that a subpopulation of p-CREB-IR cells expressed p-Trk with cystitis. These results suggest that up-regulation of p-CREB may be mediated by a neurotrophin/Trk signaling pathway.