The Journal of comparative neurology
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The intercalated cell groups, or massa intercalata, of the amygdala have been studied in rodent brains with Golgi methods. They also have been examined in gallocyanin-chromalum-, AChE-, and Timm-stained rat brains. The Golgi data indicate that the intercalated cells are not confined to a series of isolated cell clumps but form a neuronal net that covers the rostral half of the lateral-basolateral nuclear complex, stretches across a major portion of rostral amygdala, and continues rostrally beneath the anterior commissure. ⋯ It is suggested that the intercalated cells serve as sites for integration of the output of these various areas and, in turn, communicate it to the lateral-basolateral and central amygdaloid nuclei. The intercalated cells closely resemble neurons in the corpus striatum. Thus the question is raised and discussed of whether the intercalated cells are a ventral extension of the corpus striatum.
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The present study was designed to assess whether axon collateral formation and loss or retrograde cell death contribute to selective reinnervation during optic nerve regeneration in the frog, Rana pipiens. The right optic nerve was crushed in 18 frogs, and samples were taken near the optic disc (retinal segment) and near the optic chiasm (brain segment). These samples were studied quantitatively with the electron microscope at various postoperative survival times (1, 2, 3, 4, 6, 12 weeks, 6 months, 1 year; N = 2). ⋯ Autoradiography in these same animals showed the optic nerve projections normally seen after regeneration. Besides axonal loss, our results also indicate that the size of both myelinated and unmyelinated axons is significantly above normal at chronic postoperative periods. This increase in axonal size is interpreted to be related to the increased territory each remaining optic axon must fill to restore the optic projections.(ABSTRACT TRUNCATED AT 400 WORDS)
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The cell bodies of the lumbar sensory and sympathetic pre- and postganglionic neurons that project to the colon along the inferior mesenteric artery of the cat have been labeled retrogradely with horseradish peroxidase applied to the central end of their cut axons. The numbers, segmental distribution, location, and size of these labeled somata have been determined quantitatively. Afferent cell bodies were symmetrically distributed bilaterally in dorsal root ganglia T13-L5, with the maximum number (about 80%) in L3 and L4 and most of the rest in L2. ⋯ Labeled postganglionic cell bodies in both the IMG and the accessory ganglia were larger than labeled and unlabeled ganglion cells in the paravertebral ganglia. From these data, it is estimated that about 2,100 afferent neurons and about 29,000 postganglionic neurons project in the lumbar colonic nerves. In conjunction with equivalent data for the hypogastric and lumbar splanchnic nerves, the results provide a quantitative and spatial description of the afferent and efferent components of the lumbar innervation of the colon and pelvic viscera.
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Double retrograde axonal tracing experiments were carried out in order to reveal potential patterns of divergence in axonal projections from the two major sensory nuclei of the mouse brainstem trigeminal complex: the principal sensory and spinal trigeminal nuclei (oralis, interpolaris, and caudalis divisions). The tracers wheat germ agglutinin, N-[acetyl-3H] and horseradish peroxidase were used in paired injection strategies within portions of the cerebellum, superior colliculus, and thalamic ventrobasal complex and/or posterior group of adult ICR white mice. Trigeminal neurons with projections to tactile areas of the cerebellar cortex or underlying deep cerebellar nuclei were found scattered throughout the principal sensory nucleus and interpolaris division, and mainly in dorsal regions of the oralis division of the spinal trigeminal nucleus. ⋯ Double-labeled neurons were observed only after paired injections of the tracers in the thalamus and ipsilateral superior colliculus, and they were found within the caudoventral portion of the principal sensory nucleus near the oralis border, throughout the interpolaris division, within the magnocellular layer of caudalis, and only a few double-labeled neurons were also found within the marginal layer. After such injections, 50% of the labeled tectum-projecting neurons in the principal sensory nucleus, 64% in the interpolaris division, and 57% in the caudalis division are branched neurons which have collateralized projections to both the superior colliculus and thalamus. These projections, which have not been described before, appear to arise from more than one class of projection neuron which is differentially distributed within different regions of the trigeminus.
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Horseradish peroxidase was intra-axonally injected into functionally identified primary afferent fibers within the rat spinal trigeminal tract in order to study the morphology of their central terminations. They were physiologically determined to be large, myelinated, cutaneous primary afferents by means of electrical and mechanical stimulation of their receptive fields. Ninety-three axons that innervated vibrissa follicles, guard hair follicles, and slowly adapting receptors were stained for distances of 4-12 mm at the levels of the main sensory nucleus, spinal trigeminal nucleus, and rostral cervical spinal cord. ⋯ The terminal arbors of the rostral type of collaterals formed an interrupted, rostrocaudally oriented column like those seen in the lumbar dorsal horn, but the column shifted down to lamina V near the obex, and more caudally, gradually shifted upward to lamina III. Major morphological differences were not observed among the three different functional types of collaterals with respect to the rostrocaudal distribution of collaterals, and the shape and location of collaterals. The differential laminar distribution of collateral arbors of single axons along the rostrocaudal axis distinguishes the spinal trigeminal nucleus from the spinal dorsal horn where functional types of mechanoreceptive afferents form continuous or interrupted sagittal columns of terminal arbors that do not shift dorsoventrally within segments.