Problemy tuberkuleza
-
Problemy tuberkuleza · Jan 1998
Comparative Study[Diagnostic values of polymerase chain reaction test in females with genital tuberculosis].
The previously offered procedure for detecting. Mycobacterium tuberculosis, which is based on the polymerase chain reaction (PCR), has been tested for diagnosing genital tuberculosis in females. PCR was used to examine endometrial curettage specimens in 44 patients with different nosological entities, which showed a high sensitivity (80%) and a high specificity in the diagnosis of genital tuberculosis.
-
Problemy tuberkuleza · Jan 1998
Comparative Study[Specific features of DNA isolation for polymerase chain reaction in extrapulmonary tuberculosis].
While testing samples by the polymerase chain reaction (PCR), a procedure for DNA isolation is advisable adapted for different types of materials; thorough DNA purification is of the greatest importance in testing blood-containing samples, deep sample lysis is of particular importance for osseous tissue samples. A differential approach to isolating DNA from clinical samples allows the efficiency of PCR to be enhanced.
-
The authors present 7 cases with spontaneous pneumomediastinum (SPM), a rare pathological condition. The cause of SPM is rupture of marginal alveoles and spread of air into the mediastinum through perivesical and peribronchial fats. Males are more frequently affected, but females were prevalent in this study (as 4:3). ⋯ Dissection of air in all mediastinial structures, which is detectable during X-ray studies both at this stage of SPM, and at the stage of spread of air to the neck, chest, and abdomen is an important diagnostic sign. The evolution of SPM is generally good and conservative therapy leads to recovery in most patients. In 9-16% of patients, the evolution of the process is dramatic and requires cervical mediastinotomy after Tiegel or thoracotomy with wide mediastinotomy.