Journal of the Egyptian National Cancer Institute
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J Egypt Natl Canc Inst · Mar 2014
Prognostic value of IDH1 mutations identified with PCR-RFLP assay in acute myeloid leukemia patients.
Somatic mutations in isocitrate dehydrogenase 1 (IDH1) gene occur frequently in primary brain tumors. Recently theses mutations were demonstrated in acute myeloid leukemia (AML). So far, assessment of these mutations relied on the DNA sequencing technique. ⋯ IDH1 mutations are recurring genetic alterations in AML and they may have unfavorable impact on clinical outcome in adult AML. The PCR-RFLP method allows for a fast, inexpensive, and sensitive method for the detection of IDH1 mutations in AML.