Journal of the autonomic nervous system
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J. Auton. Nerv. Syst. · Jul 1998
Effect of generalised sympathetic activation by cold pressor test on cerebral haemodynamics in healthy humans.
There is no general agreement regarding several aspects of the role of the sympathetic system on cerebral haemodynamics such as extent of effectiveness, operational range and site of action. This study was planned to identify the effect of a generalised sympathetic activation on the cerebral haemodynamics in healthy humans before it is masked by secondary corrections, metabolic or myogenic in nature. A total of 35 healthy volunteers aged 20-35 underwent a 5 min lasting cold pressor test (CPT) performed on their left hand. ⋯ The increase in ABP/Vm ratio is attributed to an increase in the cerebrovascular resistance, while the concomitant reduction in PI is interpreted as due to the reduction in the compliance of the middle cerebral artery. The results suggest that generalised increases in the sympathetic discharge, causing increases in ABP, can prevent concomitant increases in CBF by acting on both small resistance and large compliant vessels. This effect is also present when a slight increase in blood pressure occurs, which suggests a moderate increase in the sympathetic discharge, i.e. when ABP remains far below the upper limit of CBF autoregulation.
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J. Auton. Nerv. Syst. · Jul 1998
Magnitude of skin vasomotor reflex represents the intensity of nociception under general anesthesia.
Because nociceptive stimuli induce the skin vasomotor reflex (SVmR), the assessment of the SVmR would be a useful indicator to represent nociception. We examined 39 adult patients for the relationship between the magnitude of the SVmR and the intensity of nociceptive stimulus that induced the SVmR. Under oxygen-nitrous oxide (50%) and sevoflurane anesthesia, the SVmR was induced by an electrical impulse to the ulnar nerve and detected by a laser Doppler flowmeter. ⋯ Study 2: under the end-tidal concentration of sevoflurane at 1.7% (n = 10), the SVmR testing was performed with a 50-mA, 50-Hz tetanic electrical impulse with the current duration changing (2, 3 or 4 s) in a randomized order. The studies demonstrated significant correlations of (1) the current intensity which induces the skin vasomotor reflex (SVmR) vs. the magnitude of the SVmR under the three different anesthesia depths, (2) the anesthesia depth vs. the magnitude of the SVmR (inverse proportion) under the same current intensity and (3) the duration of electrostimulation vs. the magnitude of the SVmR. Thus, the SVmR could be helpful for the objective assessment of nociception and anti-nociceptive effects in individual cases.
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J. Auton. Nerv. Syst. · May 1998
Calcitonin gene-related peptide and nitric oxide in the trigeminal ganglion: cerebral vasodilatation from trigeminal nerve stimulation involves mainly calcitonin gene-related peptide.
Nitric oxide (NO) is a novel neurotransmitter candidate to which a large number of physiological roles has been ascribed. In the present study, immunocytochemistry was used to demonstrate NO synthase (NOS) and to investigate possible co-localization with other neurotransmitters. In the trigeminal ganglion of the cat, a moderate number of NOS immunoreactive nerve cell bodies was seen, of which the major part also expressed calcitonin gene-related peptide (CGRP). ⋯ Local cortical administration of the CGRP blocker h-CGRP (8-37) did not alter the cerebral vasodilator response to hypercapnia or resting flow. However, the nasociliary nerve response was reduced by 50% after h-CGRP (8-37), with a general shift to the right of the frequency-response curve. These data suggest that although NOS is seen in several trigeminal ganglion cells and coexists with CGRP in a subpopulation of the sensory neurons, its role in trigeminally mediated vasodilatation was not significant.
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J. Auton. Nerv. Syst. · Mar 1998
Randomized Controlled Trial Clinical TrialEffects of naloxone on hemodynamic and sympathetic nerve responses to pain in normotensive vs. borderline hypertensive men.
Pain sensitivity decreases with increasing resting blood pressure. This blood pressure-pain interaction may be mediated by endogenous opioids which have been shown to affect both blood pressure and nociception. To test this hypothesis, we measured mean arterial blood pressure (MAP), central venous pressure (CVP), heart rate (HR), muscle sympathetic nerve activity (MSNA), serum catecholamines, and individual pain rating scales during 2 min periods of noxious mechanostimulation (skin fold pinching) in nine young (26 +/- 2 year), male normotensive (NT) subjects and in 12 age and weight matched males with borderline hypertension (BHT). ⋯ Our data do not indicate a major role of the endogenous opioid system for the blood pressure-pain interaction in man. Endogenous opioids affect pain perception and sympathetic nerve activity responses to pain in normotensive men but their activity seems to be attenuated in borderline hypertensive subjects. Therefore, the lower pain sensitivity in human essential hypertension is probably mediated by non-opioid mechanisms.
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The various transgenic strains of mice make this species an attractive experimental model. We compared qualitatively some cardiorespiratory reflexes in two different preparations of mouse: in vivo urethane anaesthetised and a working heart-brainstem preparation (WHBP). Cardiorespiratory reflexes were evoked by stimulating baroreceptors, pulmonary vagal C fibres and cardiac receptors in both preparations, while peripheral chemoreceptors were also stimulated in the WHBP. ⋯ Additionally, sodium cyanide stimulation of peripheral chemoreceptors in the WHBP produced an atropine-sensitive bradycardia and increased respiratory frequency and amplitude. Thus, the cardiorespiratory reflex responses elicited in the mouse are similar to those reported in other species. It is concluded that the qualitatively similar reflex performances between the in vivo anaesthetised mouse and the WHBP make the latter an adequate model for studying central mechanisms controlling the cardiorespiratory system.