Nihon hōigaku zasshi = The Japanese journal of legal medicine
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Nihon Hoigaku Zasshi · Oct 1996
[Personal identification using DNA polymorphism--the identification of forensic biological materials].
We performed personal identification by DNA polymorphism using forensic specimens. DNA was purified by the potassium iodine method instead of the phenol extraction method. When DNA is amplified by PCR, there are inhibitors of PCR such as melanin in hairs or blood and inorganic salts in bone. ⋯ Studies on a new microsatellite on the Y chromosome are needed for evaluation of polymorphism on the Y chromosome because it is not necessary to consider the alleles from the female in the paternity test in male children or female-derived elements in sexual delict, and there is a distance between the alleles using 27H39 alone. In this study, We evaluated the allele distribution of DYS384, DYS388, DYS389, DYS390, DYS391, DYS392, and DYS393, in Japanese subjects and found the usefulness of DYS384 and DYS390. Future studies on personal identification by DNA polymorephism will mainly evaluate short tandem repeat (STR).