Laboratory animals
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Randomized Controlled Trial
Airway management using a supraglottic airway device without endotracheal intubation for positive ventilation of anaesthetized rats.
Endotracheal intubation is often necessary for positive pressure ventilation of rats during open thoracic surgery. Since endotracheal intubation in rats is technically difficult and is associated with numerous complications, many techniques using various devices have been described in the scientific literature. In this study, we compared the effectiveness of airway management of a home-made supraglottic airway device (SAD), which is cheap to fabricate and easy to place with that of an endotracheal intubation tube in enflurane-anaesthetized rats. ⋯ Proper placement of the SAD was confirmed by observing chest wall movements that coincided with the operation of the mechanical ventilator. No complications and adverse events were encountered in the rats in which the SAD was placed, during SAD placement and immediate removal, during their mechanical ventilation through the SAD, and one week after SAD removal. From the results of blood gas analyses, we conclude that anaesthetized rats can be successfully ventilated using an SAD for open thoracic surgery.
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In biomedical research the pig is widely used as an animal model for experimental surgery. Feasible monitoring tools during anaesthesia are pivotal for successful and reliable research outcome. Blood lactate values are a monitoring tool and prognostic indicator during surgery both in humans and animals. ⋯ Considering genetic background, Rheinhybrid/Pietrain pigs (n = 51; median, 1.4 mmol/L; IQR, 1) had significantly higher blood lactate levels compared with Landrace/Pietrain crossbred animals (n = 73; median, 1.1 mmol/L; IQR, 1; P < 0.05). Body weight had no significant effect on blood lactate levels within the evaluated range. This report can benefit research projects monitoring blood lactate values in farm pigs during experimental surgery.