• Sibylle Nikolai, Patricia Huebbe, Cornelia C Metges, Anke Schloesser, Janina Dose, Naoko Ikuta, Keiji Terao, Seiichi Matsugo, and Gerald Rimbach.
• Institute of Human Nutrition and Food Science, Christian-Albrechts-University of Kiel, Germany.
• Nutrition. 2014 Feb 1; 30 (2): 228233228-33.

ObjectiveA high-fat diet (HFD) affects energy expenditure in laboratory rodents. R-α lipoic acid cyclodextrin (RALA-CD) complex is a stable form of lipoic acid (LA) and may improve energy expenditure. The aim of this study was to determine the effect of RALA-CD on energy expenditure and underlying molecular targets in female laboratory mice.MethodsFemale C57BL/6J mice were fed a HFD containing 0.1% LA for about 16 wk. The effects on energy expenditure, gene and protein expression were assessed using indirect calorimetry, real-time reverse transcriptase polymerase chain reaction, and Western blot, respectively.ResultsSupplementing mice with RALA-CD resulted in a significant increase in energy expenditure. However, both RALA per se (without γ-cyclodextrin) and S-α lipoic acid cyclodextrin did not significantly alter energy expenditure. Furthermore RALA-CD changed expression of genes encoding proteins centrally involved in energy metabolism. Transcriptional key regulators sirtuin 3 and peroxisome proliferator-activated receptor-γ, coactivator 1 alpha, as well as thyroid related enzyme type 2 iodothyronine deiodinase were up-regulated in brown adipose tissue (BAT) of RALA-CD-fed mice. Importantly, mRNA and/or protein expression of downstream effectors uncoupling protein (Ucp) 1 and 3 also were elevated in BAT from RALA-CD-supplemented mice.ConclusionOverall, present data suggest that RALA-CD is a regulator of energy expenditure in laboratory mice.Copyright © 2014 Elsevier Inc. All rights reserved.

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