• Maria Saveria Gilardini Montani, Donatella D'Eliseo, Mara Cirone, Livia Di Renzo, Alberto Faggioni, Angela Santoni, and Francesca Velotti.
• Department of Ecological and Biological Sciences, La Tuscia University, Viterbo, Italy.
• Nutrition. 2015 Apr 1; 31 (4): 578-81.

ObjectivesImmunostimulation by anticancer cytotoxic drugs is needed for long-term therapeutic success. Activation of dendritic cells (DCs) is crucial to obtain effective and long-lasting anticancer T-cell mediated immunity. The aim of this study was to explore the effect of capsaicin-mediated cell death of bladder cancer cells on the activation of human monocyte-derived CD1a+ immature DCs.MethodsImmature DCs (generated from human peripheral blood-derived CD14+ monocytes cultured with granulocyte-macrophage colony stimulating factor and interleukin-4) were cocultured with capsaicin (CPS)-induced apoptotic bladder cancer cells. DC activation was investigated using immunofluorescence and flow cytometric analysis for key surface molecules. In some experiments, CD91 was silenced in immature DCs.ResultsWe found that capsaicin-mediated cancer cell apoptosis upregulates CD86 and CD83 expression on DCs, indicating the induction of DC activation. Moreover, silencing of CD91 (a common receptor for damage-associated molecular patterns, such as calreticulin and heat-shock protein-90/70) in immature DCs led to the inhibition of DC activation.ConclusionsOur data show that CPS-mediated cancer cell apoptosis activates DCs via CD91, suggesting CPS as an attractive candidate for cancer therapy.Copyright © 2015 Elsevier Inc. All rights reserved.

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