• Arch Med Sci · Oct 2019

    MicroRNA-331-3p inhibits proliferation and metastasis of ovarian cancer by targeting RCC2.

    • Gulimire Buranjiang, Reziya Kuerban, Ailikemu Abuduwanke, Xiaowen Li, and Gulina Kuerban.
    • Department of Gynecologic Oncology Radiation Therapy (Ward II), Xinjiang Medical University Third Clinical Medical College (Affiliated Tumor Hospital), Urumqi, Xinjiang, China.
    • Arch Med Sci. 2019 Oct 1; 15 (6): 1520-1529.

    IntroductionEpithelial ovarian carcinoma (EOC) is one of the most lethal gynecologic malignancies, with a poor 5-year survival rate. Numerous studies have shown that microRNAs participate in the malignant behavior of ovarian cancer cells by directly targeting multiple oncogenes or tumor suppressor genes.Material And MethodsReverse transcription-PCR was used to determine the level of miR-331-3p in EOC. Cells proliferation was measured with the Cell Counting Kit-8. Cell mobility were measured by wound-healing assay. Cell migration and invasion were measured by transwell assay. Luciferase assays were used to demonstrate that RCC2 was a directed target of miR-331-3p in EOC. Western blots were used to measure the protein expression.ResultsWe found that the expression of microRNA-331-3p (miR-331-3p) in ovarian cancer cell lines is reduced (p < 0.01), and an increase of expression of miR-331-3p in ovarian cancer cells significantly inhibits cell proliferation (p < 0.001). Transwell and wound-healing assays showed that miR-331-3p inhibits the cell motility of ovarian cancer cells (p < 0.001). Regulator of chromosome condensation 2 (RCC2) was predicted to be a novel target for miR-331-3p. Our luciferase activity assay confirmed that RCC2 is directly targeted by miR-331-3p. RCC2 was negatively regulated by miR-331-3p (p < 0.001), and overexpression of RCC2 could restore the malignant behaviors of ovarian cancer cells, which was suppressed by miR-331-3p.ConclusionsThese data indicate that miR-331-3p can inhibit proliferation, migration, and invasion of ovarian cancer cells via directly targeting RCC2. Our study provides potential therapeutic targets for the treatment of ovarian cancer.Copyright: © 2018 Termedia & Banach.

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