• Mohammad Din, Khan M Babar, Shabir Ahmed, Abdul Aleem, Dawood Shah, Dawood Ghilzai, and Nazeer Ahmed.
• Mohammad Din PhD Scholar (Microbiology), Department of Pathology/General, Neuro and Cardiac Surgery/ Gastroenterology, Bolan Medical College/Complex Hospital, Quetta, Pakistan.
• Pak J Med Sci. 2019 Jul 1; 35 (4): 1155-1160.

ObjectiveExtensive drug resistant Gram-negative bacilli, harboring New Delhi metallo-β-lactamase-1 (bla NDM-1) having the ability to hydrolyze β-lactams, have become a vital global clinical threat. The present study was, therefore, designed to investigate the prevalence and epidemiology of NDM-1 producers in Quetta, Pakistan.MethodsThis study was carried out in Microbiology Laboratory, Bolan Medical Complex Hospital Quetta, Biotechnology laboratory, BUITEMS Quetta and Hi-tech laboratory, CASVAB, University of Balochistan, Quetta, from March to June 2018, during the hot season. Biochemical and molecular approaches were applied for the identification of bacterial isolates. Minimum Inhibitory Concentrations (MICs) were determined using E-test method. Carbapenemase activity was ascertained by Modified Hodge Test (MHT) and the presence of blaNDM-1 gene was recognized by Polymerase Chain Reaction (PCR).ResultsWe isolated five bla NDM-1 harboring isolates of three different species namely Morganella morganii (n=2) Enterobacter cloacae (n=2) and Citrobacter freundii (n=1), from 300 pus samples. These isolates were found extensive drug resistant (XDR). Strikingly, two isolates of M. morganii were displaying resistance against 23 antibiotics of sulphonamides, aminoglycosides, polypeptide, monobactams, tetracyclines, quinolones, macrolides, cephalosporins, phosphonic acid and β-lactams groups, suggesting Pan Drug Resistance (PDR).ConclusionThis is the first report on emergence of PDR strain of M. morganii producing NDM-1 in the province of Balochistan, Pakistan. The presence of bla NDM-1 in different bacterial species and their extensive rather pan drug resistance pattern poses a momentous clinical threat.

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