• José Aguirre, John M Bonvini, Barbara Rupnik, Claudio Camponovo, Andrea Saporito, and Alain Borgeat.
• From the Department of Anaesthesiology, Intensive Care and Pain Medicine, Balgrist University Hospital Zurich, Zurich (JA, JMB, BR, AB), Department of Anaesthesiology, Clinica Ars Medica, Gravesano (CC), Department of Anaesthesiology, Ospedale Regionale di Bellinzona e Valli, Bellinzona, Switzerland (AS) and Department of Surgery, University of Illinois at Chicago, Chicago, Illinois, USA (AB).
• Eur J Anaesthesiol. 2021 Mar 1; 38 (Suppl 1): S24S32S24-S32.

BackgroundDiclofenac and other NSAIDs are routinely used in the postoperative period. Their effect on fracture healing remains unclear and controversial.ObjectiveThe primary outcome was to assess the potential cytotoxicity of clinically relevant concentrations of diclofenac on human osteoblasts.DesignLaboratory in vitro study.SettingInstitute of Physiology, Zurich, Center for Integrative Human Physiology, University of Zurich.MaterialsMonolayers of human osteoblasts.InterventionsExposure of human osteoblast monolayers to several concentrations of diclofenac, for different periods of time, with and without an artificially induced inflammatory process.Main Outcome MeasuresCell count, cell viability, cell proliferation and apoptosis.ResultsA concentration-mediated, time and exposure dependent cytotoxic effect of diclofenac-mediated apoptosis was observed. Stimulated inflammatory conditions seemed to reduce toxic effects.ConclusionCytotoxic effects of diclofenac are exposure, time and concentration dependent. Simulating aspects of inflammatory conditions seems to increase resistance to diclofenac cytotoxicity, especially in the presence of higher concentration and longer exposure time.Copyright © 2020 European Society of Anaesthesiology and Intensive Care. Unauthorized reproduction of this article is prohibited.

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