• Transl Res · May 2021

    The role of the lectin pathway of the complement system in SARS-CoV-2 lung injury.

    • Mineia Alessandra Scaranello Malaquias, Ana Carolina Gadotti, Jarbas da Silva Motta-Junior, Ana Paula Camargo Martins, Marina Luise Viola Azevedo, Ana Paula Kubaski Benevides, Plínio Cézar-Neto, Letícia Arianne Panini do Carmo, Rafaela Chiuco Zeni, Sonia Mara Raboni, Aline Simoneti Fonseca, Cleber Machado-Souza, Andrea Novais Moreno-Amaral, and Lucia de Noronha.
    • Postdoctoral Researcher Postgraduate Program in Health Sciences School of Medicine, Pontifícia Universidade Católica do Paraná - PUCPR, Curitiba, PR, Brazil. Electronic address: andrea.moreno@pucpr.br.
    • Transl Res. 2021 May 1; 231: 556355-63.

    AbstractAlthough some evidence showed the activation of complement systems in COVID-19 patients, proinflammatory status and lectin pathway remain unclear. Thus, the present study aimed to demonstrate the role of MBL and ficolin-3 in the complement system activation and compared to pandemic Influenza A virus H1N1 subtype infection (H1N1pdm09) and control patients. A total of 27 lungs formalin-fixed paraffin-embedded samples (10 from H1N1 group, 6 from the COVID-19 group, and 11 from the control group) were analyzed by immunohistochemistry using anti-IL-6, TNF-alfa, CD163, MBL e FCN3 antibodies. Genotyping of target polymorphisms in the MBL2 gene was performed by real-time PCR. Proinflammatory cytokines such as IL-6 and TNF-alpha presented higher tissue expression in the COVID-19 group compared to H1N1 and control groups. The same results were observed for ICAM-1 tissue expression. Increased expression of the FCN3 was observed in the COVID-19 group and H1N1 group compared to the control group. The MBL tissue expression was higher in the COVID-19 group compared to H1N1 and control groups. The genotypes AA for rs180040 (G/A), GG for rs1800451 (G/A) and CC for rs5030737 (T/C) showed a higher prevalence in the COVID-19 group. The intense activation of the lectin pathway, with particular emphasis on the MBL pathway, together with endothelial dysfunction and a massive proinflammatory cytokines production, possibly lead to a worse outcome in patients infected with SARS-Cov-2. Moreover, 3 SNPs of our study presented genotypes that might be correlated with high MBL tissue expression in the COVID-19 pulmonary samples.Copyright © 2020 Elsevier Inc. All rights reserved.

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