• Sondes Haddad-Boubaker, Marwa Lakhal, Cyrine Fathallah, Samar Mhimdi, Aida Bouafsoun, Amel Kechrid, and Hanen Smaoui.
• Laboratory of Microbiology of Bechir Hamza Children's Hospital, LR18ES39, Faculty of Medicine of Tunis, Tunis El-Manar University, Tunis, Tunisia.
• Afr Health Sci. 2020 Sep 1; 20 (3): 1124-1132.

BackgroundSince the 1990s, the epidemiology of bacterial meningitis worldwide has changed thanks to vaccination. In Tunisia, the main causative pathogens were Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae serotype b (Hib). Only Hib vaccination was available during our study period.ObjectivesWe performed a laboratory case report based-study of suspected bacterial meningitis in Northern Tunisia from January 2014 to June 2017.MethodsCSF samples obtained from children beyond neonatal age with suspicion of meningitis were tested by two real time PCRs, targeting pneumococcus, meningococcus and Hib, and conventional methods.ResultsUsing real-time PCR, 63 were positive including ten supplementary cases compared to conventional methods. A general decrease of bacterial meningitis cases was demonstrated comparing to previous data. Pneumococcus was predominant (69.84%) followed by meningococcus (28.57%) and Hib (1.59%). The main serotypes were 14, 19F, 6B and 23F for pneumococcus and serogroup B for meningococcus. Most cases occurred during cold season and children under one year were the most affected by bacterial meningitis.ConclusionOur study suggests the predominance of pneumococcal cases. It may provide valuable data on meningitis epidemiology before the introduction of pneumococcal vaccine, which may be useful for future evaluation.© 2020 Haddad-Boubaker S et al.

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