• Zhonghua Yu Fang Yi Xue Za Zhi · Mar 2004

    Comparative Study

    [Detection for severe acute respiratory syndrome (SARS) coronavirus RNA in stool of SARS patients].

    • Zhong-ping He, Qing-ming Dong, Shu-jing Song, Lin He, and Hui Zhuang.
    • Research Center of Virology, Beijing Ditan Hospital, Beijing 100011, China.
    • Zhonghua Yu Fang Yi Xue Za Zhi. 2004 Mar 1; 38 (2): 90-1.

    ObjectiveTo investigate excretion of severe acute respiratory syndrome coronavirus RNA (SARS-CoV) in stool of SARS patients.MethodsSARS-CoV RNA was detected in stool specimens with fluorescent quantitative polymerase chain reactions (FQ-PCR) in 101 SARS patients on the 10 to 55 days after onset, 27 non-SARS patients and 400 individuals with health check-up.ResultsSARS-CoV RNA was positive in stool specimens by FQ-PCR in 58 of 101 SARS patients (57.4%), and all negative in 27 non-SARS patients and 400 healthy individuals. Positive rate of SARS-CoV RNA was 100% (8/8), 67.7% (21/31), 47.4% (27/57) and 40.0% (2/5) on the 10 - 19, 20 - 29, 30 - 39 and 40 - 55 days after onset of fever, respectively, with values of logarithm of SARS-CoV RNA load of 6.06 +/- 2.05, 4.51 +/- 1.23, 3.82 +/- 1.44 and 3.57 +/- 1.25, respectively.ConclusionPositive rate and load of SARS-CoV RNA in stool of SARS patients was the highest at their acute phase, and decreased with the extension of its course.

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