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Acta Biochim. Biophys. Sin. (Shanghai) · Nov 2004
Accumulation of pathogenesis-related type-5 like proteins in phytoplasma-infected garland chrysanthemum Chrysanthemum coronarium.
- Bo-Xiong Zhong and Yan-Wei Shen.
- Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China. bxzhong@zju.edu.cn
- Acta Biochim. Biophys. Sin. (Shanghai). 2004 Nov 1; 36 (11): 773-9.
AbstractSoluble proteins extracted from leaves, apical shoots, axillary shoots, and stems of garland chrysanthemum plants infected by onion yellows phytoplasma were analyzed by two-dimensional gel electrophoresis. Computerized matching analysis revealed that at least six soluble proteins were accumulated specifically in phytoplasma-infected garland chrysanthemum. N-terminal amino acids sequences of these soluble proteins, determined by Edman degradation, shared high sequence similarities with those of pathogenesis-related type-5 (PR-5) proteins such as tobacco thaumatin-like protein. Accumulation of these six proteins was also found in garland chrysanthemum plants infected by other phytoplasmas. These results demonstrate that phytoplasmal infection induces the accumulation of PR-5 like proteins in garland chrysanthemum plants.
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