• Amir Sotoudeh, Mohammad Ashrafzadeh Takhtfooladi, Amirali Jahanshahi, Adel Haghighi Khiabanian Asl, Hamed Ashrafzadeh Takhtfooladi, and Mohammadreza Khansari.
• Faculty of Veterinary Science, Kahnooj Branch, Islamic Azad University, Kerman, Iran.
• Acta Cir Bras. 2012 Feb 1; 27 (2): 168-71.

PurposeTo investigate whether N-acetylcysteine, a free radicals scavenger has a protective effect against lung injury as a remote organ after skeletal muscle ischemia-reperfusion.MethodsTwenty Wistar male rats were divided randomly into two experimental groups: group ischemia-reperfusion (group I) and group ischemia-reperfusion +N-acetylcysteine (group II). All animals were undergone two hours of ischemia by occlusion femoral artery and 24h of reperfusion. Before clamped the femoral artery, 250 IU heparin was administered via the jugular vein to prevent clotting. Rats that were treated with N-acetylcysteine given IV at a dose of 150 mgkg(-1), immediately before reperfusion. After 24h of reperfusion, animals were euthanized and left lung harvested for histopathological analysis under light microscopy.ResultsIn the group I, tissues showed histological changes with intra-alveolar edema, intra-alveolar hemorrhage and neutrophilic infiltration. Histopathologically, there was a significant difference (P = 0.005) between two groups.ConclusionAdministration of N-acetylcysteine treatment significantly decreased lung injury induced by skeletal muscle ischemia reperfusion according to histological findings.

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