• Gisele Graça Leite Dos Santos, Anna Lethícia Lima Oliveira, Dourivaldo Silva Santos, Renan Fernandes do Espírito Santo, Daniela Nascimento Silva, Paulo José Lima Juiz, Soares Milena Botelho Pereira MBP Gonçalo Moniz Institute, Oswaldo Cruz Foundation-FIOCRUZ, 40296-710 Salvador, BA, Brazil; SENAI Institute of Innovation in Advanced Heal, and Cristiane Flora Villarreal.
• Gonçalo Moniz Institute, Oswaldo Cruz Foundation-FIOCRUZ, 40296-710 Salvador, BA, Brazil.
• Life Sci. 2021 Jan 15; 265: 118755.

AimsThe present study was designed to investigate whether the antinociceptive effect of bone marrow-derived mesenchymal stem/stromal cells (MSC) during oxaliplatin (OXL)-induced sensory neuropathy is related to antioxidant properties.Main MethodsMale mice C57BL/6 were submitted to repeated intravenous administration of OXL (1 mg/kg, 9 administrations). After the establishment of sensory neuropathy, mice were treated with a single intravenous administration of MSC (1 × 106), vehicle or gabapentin. Paw mechanical and thermal nociceptive thresholds were evaluated through von Frey filaments and cold plate test, respectively. Motor performance was evaluated in the rota-rod test. Gene expression profile, cytokine levels, and oxidative stress markers in the spinal cord were evaluated by real-time PCR, ELISA and biochemical assays, respectively.Key FindingsOXL-treated mice presented behavioral signs of sensory neuropathy, such as mechanical allodynia and thermal hyperalgesia, which were completely reverted by a single administration of MSC. Repeated oral treatment with gabapentin (70 mg/kg) induced only transient antinociception. The IL-1β and TNF-α spinal levels did not differ between mice with or without sensory neuropathy. MSC increased the levels of anti-inflammatory cytokines, IL-10 and TGF-β, in the spinal cord of neuropathic mice, in addition to increasing the gene expression of antioxidant factors SOD and Nrf-2. Additionally, nitrite and MDA spinal levels were reduced by the MSC treatment.SignificanceMSC induce reversion of sensory neuropathy induced by OXL possibly by activation of anti-inflammatory and antioxidant pathways, leading to reestablishment of redox homeostasis in the spinal cord.Copyright © 2020 Elsevier Inc. All rights reserved.

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