• Logan J Voss, Cecilia Hansson Baas, Linnea Hansson, D Alistair Steyn-Ross, Moira Steyn-Ross, and James W Sleigh.
• Waikato District Health Board, Anaesthesia Department, Pembroke Street, Hamilton 3204, New Zealand. vossl@waikatodhb.health.nz
• Eur. J. Pharmacol. 2012 Aug 15; 689 (1-3): 111-7.

AbstractGeneral anaesthetics have been hypothesised to ablate consciousness by decoupling intracortical neural connectivity. We explored this by investigating the effect of etomidate and ketamine on coupling of neural population activity using the low magnesium neocortical slice model. Four extracellular electrodes (50 μm) were positioned in mouse neocortical slices (400 μm thick) with varying separation. The effect of etomidate (24 μM) and ketamine (16 μM) on the timing of population activity recorded between channels was analysed. No decoupling was observed at the closest electrode separation of 0.2 mm. At 4mm separation, decoupling was observed in 50% and 42% of slices during etomidate and ketamine delivery, respectively (P<0.0001 and P=0.002, compared to 0.2 mm separation). A lower rate of decoupling was observed with 1mm separation (21% and 8%, respectively, P<0.03 for etomidate compared to 0.2mm separation). The data support the hypothesis that mechanistically diverse general anaesthetics disrupt neuronal connectivity across widely distributed intracortical networks.Copyright © 2012 Elsevier B.V. All rights reserved.

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