• Feda S Aljaser, Hazem K Ghneim, Mashael M ALshubaily, Manal Abudawood, Faisal Almajed, Sabiha Fatima, and Yazeed A ALsheikh.
• From the Department of Clinical Laboratory Sciences and the Chair of Medical and Molecular Genetics Research (Aljaser, Ghneim, Abudawood, Fatima, ALsheikh), College of Applied Medical Sciences, King Saud University; from the Department of Obstetrics and Gynecology (ALshubaily), College of Medicine, King Saud University; from the Department of Clinical Laboratory Sciences (Almajed), College of Applied Medical Sciences, King Saud Bin Abdulaziz University for Health Sciences, Riyadh, Kingdom of Saudi Arabia.
• Saudi Med J. 2021 May 1; 42 (5): 491-498.

ObjectivesTo assess the oxidation state and gene expression profiles of relevant enzymes in intrauterine growth restriction (IUGR) patients in Saudi Arabia.MethodsCurrent case-control study involved plasma and placental tissue samples from 25 IUGR patients and 25 healthy pregnant (HP) women attending the Obstetrics and Gynecology Clinic, King Khalid University Hospital, Riyadh, Saudi Arabia, between April and November 2017. We compared hydrogen peroxide, superoxide anions, malondialdehyde, and oxidative stress markers levels and the activities of glutathione-related enzymes (glutathione peroxidase [GPx], glutathione reductase [GR], glutathione S-transferase [GST], glutamate cysteine ligase [GCL], glutathione synthetase [GS], reduced glutathione [GSH], oxidized glutathione [GSSG], and oxidized nicotinamide adenine dinucleotide [NAD+], and reduced NAD [NADH]) between the 2 groups. We also compared differential expression levels of glutathione-related enzyme genes using reverse transcription-quantitative polymerase chain reaction.ResultsOxidative stress markers significantly differed in IUGR samples, while GSH levels and GPx, GR, GST, GCL, and GS activities and their placental mRNA transcriptional levels were significantly lower. Plasma and placental NAD+ levels were also significantly lower, while NADH levels were significantly higher, causing lowered NAD+-NADH ratios in the IUGR group compared to control.ConclusionsIntrauterine growth restriction patients show a metabolic shift in favor of oxidation compared to HP women.Copyright: © Saudi Medical Journal.

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