• Emerg Microbes Infect · Dec 2020

    Letter

    Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing.

    • Fabiola Mancini, Fabrizio Barbanti, Maria Scaturro, Giulia Errico, Angelo Iacobino, Antonino Bella, Flavia Riccardo, Giulia Marsili, Paola Stefanelli, Patrizio Pezzotti, Giovanni Rezza, Alessandra Ciervo, and on behalf ISS COVID-19 study group.
    • Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.
    • Emerg Microbes Infect. 2020 Dec 1; 9 (1): 1393-1396.

    AbstractThe RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.

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