• M A Cousin, H Hurst, and D G Nicholls.
• Department of Pharmacology, Ninewells Medical School, University of Dundee, U.K.
• Neuroscience. 1997 Nov 1; 81 (1): 151-61.

AbstractRegulated exocytosis from cultured rat cerebellar granule cells can be localized by the vesicle specific marker FM2-10 to specific sites, the highest density of which are at visible varicosities coinciding with neurite-neurite contacts. Exocytosis can be evoked by uniform electrical field pulses, which initiate tetrodotoxin-sensitive action potentials, or by elevated KCl. [3H]D-Aspartate is an authentic false transmitter in this preparation, judged by sensitivity of release to bafilomycin A1 and tetanus toxin. The coupling of presynaptic voltage-activated Ca2+ channels to [3H]D-aspartate exocytosis was determined during field stimulation. The peak cytoplasmic free Ca2+ concentration achieved in the varicosities was proportional to Ca2+ entry during a 10 strain of pulses. L-type Ca2+ channels did not contribute to either Ca2+ entry or [3H]D-aspartate exocytosis. The P-type Ca2+ channel antagonist omega-agatoxin-IVA (30 nM) only inhibited at 75% of the varicosities, although a mean 15% inhibition of Ca2+ entry caused a 39% inhibition of exocytosis. In contrast the N-type Ca2+ channel inhibitor omega-conotoxin-GVIA (1 microM), which inhibited at virtually all varicosities, caused mean inhibitions of Ca2+ entry and exocytosis of 26% and 24% respectively. The toxin omega-conotoxin-MVIIC (5 microM), which inhibits N-, P- and Q-type Ca2+ channels, was effective at all varicosities. The Q-type component of Ca2+ entry was calculated to be only 5-10%; however, the additional inhibition of exocytosis was 30%. Thus P-type and particularly Q-type channels appear to be more closely coupled to exocytosis than N-type Ca2+ channels. The residual Ca2+ entry following 5 microM omega-conotoxin-MVIIC is scarcely coupled to release. The omega-agatoxin-IVA and omega-conotoxin-GVIA inhibitions of both Ca2+ entry and exocytosis were additive and varied stochastically between individual varicosities. These results demonstrate that both Q- and P-type Ca2+ channels are highly efficient in their coupling to amino acid exocytosis, with N-type less efficient, and L-type channels not at all. The Ca2+ channel types coupled to exocytosis are also able to support exocytosis when evoked by either brief field-evoked action potentials or prolonged depolarization with KCl, indicating that these presynaptic channels, in contrast to those on the somata of the cells, can respond to widely different patterns of activation.

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