• Kihwang Lee, Young Lee Sun S, So Yean Park, and Hongseok Yang.
• Department of Ophthalmology, Ajou University School of Medicine , Suwon , Republic of Korea.
• Curr. Eye Res. 2014 Jul 1; 39 (7): 680-5.

ObjectiveThe effects of pirfenidone were investigated on cultured human pterygium fibroblasts (HPFs).MethodsHPFs were obtained from pterygium surgery and subjected to primary culture. After treatment with 0.5, 1.0 or 1.5 mg/mL pirfenidone, MTT and cell migration assays were performed, and procollagen secretion and TGF-β expression were measured by Western blotting and immunofluorescence analysis.ResultsPirfenidone had a significant inhibitory effect on HPF proliferation, migration and collagen synthesis. There were no differences between the cells treated with 0.5, 1.0 and 1.5 mg/mL pirfenidone and the controls in the MTT assay. After 48 h of treatment with 1.0 or 1.5 mg/mL pirfenidone, TGF-β expression was significantly decreased.ConclusionsThese findings demonstrate that pirfenidone inhibits the proliferation, migration and procollagen secretion of HPFs at nontoxic concentrations by decreasing TGF-β expression. Thus, pirfenidone may be considered as a safe adjuvant for pterygium surgery to prevent recurrence.

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