• Methods Mol. Biol. · Jan 2018

    Straightforward Protocol for Gel-Free Proteomic Analysis of Adipose Tissue.

    • Yvonne Pasing, Armin Schniers, and Terkel Hansen.
    • Tromsø Endocrine Research Group, Department of Clinical Medicine, UiT - The Arctic University of Norway, Tromsø, Norway.
    • Methods Mol. Biol. 2018 Jan 1; 1788: 289-296.

    AbstractAfter conducting systematic and quantitative comparisons of different sample preparation techniques regarding their capability to efficiently and reproducibly recover proteins from biopsies, we present here our superior protocol for extracting proteins from low amounts of adipose tissue. Adipose tissue as a matrix in bottom-up proteomics is challenging due to the extremely high lipid content.The lysis buffer utilized contains the detergent sodium deoxycholate, which does not impair the activity of trypsin and therefore enables direct digestion without detergent removal steps. The resulting workflow is time saving, cost efficient, easy to perform, and it can also be applied to other hydrophobic samples.

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