• Fleur Wolff, Hafid Dahma, Cécile Duterme, Sigi Van den Wijngaert, Olivier Vandenberg, Frédéric Cotton, and Isabel Montesinos.
• Department of Clinical Chemistry, Laboratoire Hospitalier Universitaire de Bruxelles (LHUB-ULB), Université Libre de Bruxelles (ULB), Brussels, Belgium. Electronic address: Fleur.wolff@LHUB-ULB.be.
• Diagn. Microbiol. Infect. Dis. 2020 Nov 1; 98 (3): 115140.

IntroductionSARS-CoV-2 seroconversion is important for epidemiological studies as well as contact tracing.Material And MethodsThe antibody response against SARS-CoV-2 was examined in 111 patients with a positive qRT-PCR. Seroconversion was assessed using the Elecsys from Roche, the Liaison S1/S2 IgG from Diasorin, the IgG and IgA from Euroimmun, as well as the VIDAS IgG and IgM. Specificity was estimated based on the measurement of SARS-CoV-2 antibodies in 96 residual samples collected during a non-pandemic period.ResultsThe highest overall sensitivity for detecting seroconversion was obtained using the Elecsys (81.1%), the Euroimmun with a combined detection of IgG/IgA (86.5%), and the VIDAS with a simultaneous measurement of IgG/IgM (78.4%).The Elecsys and the VIDAS IgG/IgM demonstrated a specificity as well as a positive predictive value of 100%.ConclusionsThe Elecsys and the VIDAS methods with a combination of IgG/IgM measurement demonstrated a high sensitivity with no false positive results.Copyright © 2020 Elsevier Inc. All rights reserved.

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