• Yasuo Hosaka, Atsushi Saito, Ryo Maeda, Chie Fukaya, Satoru Morikawa, Asako Makino, Kazuyuki Ishihara, and Taneaki Nakagawa.
• Department of Dentistry and Oral Surgery, School of Medicine, Keio University, 35 Shinanomachi, Sinnjuku-ku, Tokyo, Japan.
• Arch. Oral Biol. 2012 Apr 1; 57 (4): 364-8.

ObjectiveTo investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens.DesignPorphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72 h, PVP-I solutions were applied to the biofilm for the time period ranging from 0.5 to 5 min. After addition of a deactivator, each PCM was removed and the biofilm on the PCM was serially diluted and plated on blood agar plates and cultured anaerobically for 7 days. Then viable bacteria were enumerated.ResultsIn the dual species biofilm model, F. nucleatum showed an approximately 200-fold increase in viable counts when compared with mono-microbial biofilm. In dual species biofilm, PVP-I with concentration equal to or greater than 2% was required to significantly reduce P. gingivalis and F. nucleatum. When the contact time of PVP-I was increased to 1 min or greater, no difference in antibacterial activity of PVP-I was observed in any concentration.ConclusionThese results suggest that 30s application of 2% PVP-I would be effective in suppressing both P. gingivalis and F. nucleatum in dual-species biofilm, and this provides clinical implication for the control of subgingival biofilm.Copyright © 2011 Elsevier Ltd. All rights reserved.

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