-
- Alyson A Kelvin, Norbert Degousee, David Banner, Eva Stefanski, Alberto J Leόn, Denis Angoulvant, Stéphane G Paquette, Stephen S H Huang, Ali Danesh, Clinton S Robbins, Hossein Noyan, Mansoor Husain, Gerard Lambeau, Michael Gelb, David J Kelvin, and Barry B Rubin.
- Immune Diagnostics & Research, Toronto, Ontario, Canada.
- Virology. 2014 Apr 1;454-455:78-92.
AbstractThe role of Group X secreted phospholipase A2 (GX-sPLA2) during influenza infection has not been previously investigated. We examined the role of GX-sPLA2 during H1N1 pandemic influenza infection in a GX-sPLA2 gene targeted mouse (GX(-/-)) model and found that survival after infection was significantly greater in GX(-/-) mice than in GX(+/+) mice. Downstream products of GX-sPLA2 activity, PGD2, PGE2, LTB4, cysteinyl leukotrienes and Lipoxin A4 were significantly lower in GX(-/-) mice BAL fluid. Lung microarray analysis identified an earlier and more robust induction of T and B cell associated genes in GX(-/-) mice. Based on the central role of sPLA2 enzymes as key initiators of inflammatory processes, we propose that activation of GX-sPLA2 during H1N1pdm infection is an early step of pulmonary inflammation and its inhibition increases adaptive immunity and improves survival. Our findings suggest that GX-sPLA2 may be a potential therapeutic target during influenza.Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.
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