• Jian Zaiyou, Wang Hongsheng, Wang Ning, Meng Li, and Xu Guifang.
• Henan Institute of Science and Technology, Xinxiang. Collaborative Innovation Center of Modern Biological Breeding, Xinxiang.. jian19732004@126.com.
• Nutr Hosp. 2015 Dec 1; 32 (6): 2932-7.

AbstractThe objective of this study was to isolate endophytic fungi producing paclitaxel from yew for the purpose of paclitaxel manufacture. Surface sterilized bark of Taxus wallichiana var. mairei was used as source material and potato dextrose agar culture medium was used in isolation of endophytic fungi. Fungal cultures were extracted with a mixture of chloroform / methanol (1:1, v/v) and the paclitaxel in the extracts was determined and authenticated with LC-MS. An endophytic fungus that produced paclitaxel was identified by ITS rDNA and 26S D1/D2 rDNA sequencing. The results showed that a total of 435 endophytic fungal strains were isolated from T. wallichiana var. mairei and purified. Only one of these strains produced paclitaxel and it belongs to Fusarium. The paclitaxel productivity in whole PDB culture and that in spent culture medium from this strain is 0.0153 mg/L and 0.0119 mg/L respectively. The paclitaxel content in dry mycelium is 0.27 mg/kg. This isolated endophytic fungus produced paclitaxel at a considerable level and shows potentiality as a producing strain for paclitaxel manufacture after strain improvement. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.

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