• Binjian Liu, Xin Lü, Chaoling Qi, Shuhui Zheng, Muxiu Zhou, Jianmin Wang, and Wen Yin.
• Laboratory department, 161st Central Hospital of P.L.A., Wuhan 430010, Hubei Province, China.
• Afr Health Sci. 2014 Sep 1; 14 (3): 648-56.

BackgroundBinding of keratinocyte growth factor (KGF) to the KGF receptor (KGFR) plays an important role in the recovery of alveolar epithelial cells from acute lung injury (ALI).ObjectivesTo evaluate the effect of gene therapy via adenovirus gene transfer of KGFR on the treatment of ALI.MethodsSprague-Dawley rats were divided into four groups: normal controls, injury controls, normal adenovirus transduced group and injury adenovirus transduced group. The ALI model was induced by lipopolysaccharide (LPS) injection. Recombinant adenovirus (AdEasy-KGFR) was injected via the tail vein. Expression of the sodium (Na(+)) channel in rat alveolar type II (ATII) epithelial cells was determined by PCR, immunohistochemistry and immunoelectron microscopy of rat lung tissues.ResultsGene expression of the Na(+) channel and KGFR in ATII cells was higher in the normal adenovirus transduced group than the three other groups; expression of these two genes in the injury adenovirus transduced group was higher than the injury control group. Na(+) channel protein expression was lower in the injury adenovirus transduced group but higher than the injury control group.ConclusionsKGFR over-expression induced Na channel expression could potentially be beneficial for ALI therapy.

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