• Saudi Med J · Dec 2021

    The use of ex-vivo liver perfusion circuit in sheep model: Preliminary report.

    • Mohammed I Al-Sebayel, Yasser M El-Sheikh, Falah H Al-Mohanna, Saleh I Al Abbad, Yaser H Al Nemry, Ahmed Al-Jammali, Norah K Al-Zeer, Yazeed M Alsebayel, and Hamad M Al-Bahli.
    • From the Department of Liver Transplantation & Hepatobiliary-Pancreatic Surgery (Al-Sebayel, El-Sheikh, Al Abbad, Al Nemry, Al-Jammali, Al-Zeer, Alsebayel, Al-Bahli); and from the Department of Compara... more tive Medicine (Al-Mohanna), King Faisal Specialist Hospital & Research Center, Riyadh, Kingdom of Saudi Arabia. less
    • Saudi Med J. 2021 Dec 1; 42 (12): 1289-1295.

    ObjectivesTo describe a novel animal model for ex-vivo liver perfusion.MethodsThis study was carried out at King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia, between September 2016 and January 2019. We assembled a perfusion circuit operated by a continuous pressure-driven arterial pump with continuous portal and arterial pressure and volume measurements. We used normothermic oxygenated perfusate. The livers used were retrieved from the sheep.ResultsEx-vivo continuous perfusion of the liver was achieved for up to 9 hours with stable pressure and volume in both hepatic artery and portal vein. In 4 experiments the arterial pressure was kept in a range of 48-52 mmHg with a mean of 51.75±4.31 resulting in arterial volume at steady state of 223.5±48.25 ml/minute (95% confidence level). At steady state the mean portal pressure was 16.25±1.45 mmHg with a mean volume of 854±313.75 ml/minute (95% confidence level). Bile production was observed during the perfusion period. Hemodynamic parameters were similar to the physiological parameters observed in normothermic perfusion model of the porcine liver.ConclusionA normothermic oxygenated ex-vivo perfusion circuit was successfully constructed using the sheep liver. A sustainable functional circuit with physiological hemodynamic parameters was achieved. Further study on sheep model seems to be feasible.Copyright: © Saudi Medical Journal.

      Pubmed     Free full text   Copy Citation     Plaintext  

      Add institutional full text...

    Notes

     
    Knowledge, pearl, summary or comment to share?
    300 characters remaining
    help        

    hide…

What will the 'Medical Journal of You' look like?

Start your free 21 day trial now.

We guarantee your privacy. Your email address will not be shared.