• İskender Kaplanoğlu, Take KaplanoğluGülnurG0000-0002-3661-3488Department of Histology and Embryology, Faculty of Medicine, Gazi University, Ankara, Turkey, Özgür Çınar, Güleser Göktaş, Serdar Dilbaz, and Cemile Merve Seymen.
• Center of Assisted Reproduction, Etlik Zübeyde Hanım Women’s Health Teaching and Research Hospital, Sağlık Bilimleri University Ankara, Turkey
• Turk J Med Sci. 2019 Aug 8; 49 (4).

Background/AimA synchronized dialogue between maternal and embryonic tissues is required for successful implantation. Low uterine receptivity is responsible for two-thirds of implantation failures and leptin is effective in the physiology of reproduction by binding to specific receptors. In this study, we investigateleptin receptor expression in cases of embryo transfer to endometrial coculture.Materials And MethodsBiopsy materials were taken from 20 females with indication for coculture application and were cultured in an appropriate medium after the epithelial cells were isolated. The grown cells were cultured in chamber slides as the first group. For the second group, day 3 embryo was added to chamber slides and the development was observed. The embryo was transferred 1 or 2 days later and other cells (after the transfer process) were used to form the second group. After fixation, immunohistochemical staining was performed with anti-leptin primary antibody.ResultsRegarding the coculture without embryo transfer, moderate leptin receptor immunoreactivity was seen in the perinuclear region and the cell membrane. Also, regarding the coculture with embryo transfer, moderate leptin receptor immunoreactivity was seen in the cytoplasm and strong leptin receptor immunoreactivity was seen in the cell membrane.ConclusionEmbryo transfer to endometrium coculture triggers leptin receptor expressionThis work is licensed under a Creative Commons Attribution 4.0 International License.

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