• J. Med. Virol. · Apr 2021

    COVID-19 screening test by using random oropharyngeal saliva.

    • Mohan Rao, Fairuz A Rashid, Fashihah S A H Sabri, Nur Nadia Jamil, Valentinus Seradja, Nurul A Abdullah, Hanisah Ahmad, S L Aren, Shareh A S Ali, Mawaddah Ghazali, Anizan A Manaf, Harishah Talib, Rohaidah Hashim, Rozainanee Zain, Ravindran Thayan, Fairuz Amran, Tahir Aris, and Norazah Ahmad.
    • Infectious Disease Research Centre, Institute for Medical Research, Ministry of Health Malaysia, Jalan Pahang, Kuala Lumpur, Malaysia.
    • J. Med. Virol. 2021 Apr 1; 93 (4): 2461-2466.

    AbstractAn optimal clinical specimen for accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by minimizing the usage of consumables and reduce hazard exposure to healthcare workers is an urgent priority. The diagnostic performance of SARS-CoV-2 detection between healthcare worker-collected nasopharyngeal and oropharyngeal (NP + OP) swabs and patient performed self-collected random saliva was assessed. Paired NP + OP swabs and random saliva were collected and processed within 48 h of specimen collection from two cohort studies which recruited 562 asymptomatic adult candidates. Real-time reverse-transcription polymerase chain reaction targeting Open reading frame 1a (ORF1a) and nucleocapsid (N) genes was performed and the results were compared. Overall, 65 of 562 (28.1%) candidates tested positive for COVID-19 based on random saliva, NP + OP swabs, or both testing techniques. The detection rate of SARS-CoV-2 was higher in random saliva compared to NP + OP testing (92.3%; 60/65 vs. 73.8%; 48/65; p < .05). The estimated sensitivity and specificity of random saliva were higher than NP + OP swabs (95.0; 99.9 vs. 72.2; 99.4). The Ct  values of ORF1a and N genes were significantly lower in random saliva compared to NP + OP swabs specimens. Our findings demonstrate that random saliva is an alternative diagnostic specimen for the detection of SARS-CoV-2. Self-collected random oropharyngeal saliva is a valuable specimen that provides accurate SARS-CoV-2 surveillance testing of a community.© 2021 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC.

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