• Gac Med Mex · Jan 2021

    High frequency of MLH1 promoter methylation mediated by gender and age in colorectal tumors from Mexican patients.

    • José M Moreno-Ortiz, Josselyn Jiménez-García, Melva Gutiérrez-Angulo, Ma de la Luz Ayala-Madrigal, Anahí González-Mercado, Christian O González-Villaseñor, Beatriz A Flores-López, Carlos Alvizo-Rodríguez, Jesús A Hernández-Sandoval, Martha A Fernández-Galindo, Víctor Maciel-Gutiérrez, Helen Ramírez-Plascencia, and Ruth Ramírez-Ramírez.
    • Department of Molecular Biology and Genomics, Dr. Enrique Corona Rivera Human Genetics Institute, Health Sciences University Center, Universidad de Guadalajara.
    • Gac Med Mex. 2021 Jan 1; 157 (6): 618-623.

    IntroductionSeveral genes determine the development of colorectal cancer (CRC), such as MLH1, which encodes a protein that participates in DNA repair. MLH1 hypermethylation has been associated with gene silencing.ObjectiveTo analyze the methylation of five regions of MLH1 CpG island in colorectal tumors from Mexican patients.Materials And MethodsOne hundred and one tumor tissue samples were obtained from Mexican patients with CRC who provided informed consent. DNA was subjected to bisulfite conversion. Methylation of all five regions of the CpG island was evaluated using methylation-specific PCR.ResultsThe frequency of methylation in Mexican patients with CRC was 25%. Regions A and B methylation was the main observed pattern (60%). Female patients showed a higher frequency of methylation (71%; OR 3.085; CI: 1.85-8.03; p = 0.02), and out of total methylated samples, 80% corresponded to individuals older than 45 years (p < 0.05).ConclusionWe calculated a methylation frequency for the MLH1 gene of 25% in Mexican patients with CRC, with this being the first report for this population. Female patients and patients older than 45 years showed a higher frequency of methylation.Copyright: © 2021 Permanyer.

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