• Wei-Wei Ma, Bing-Jie Ding, Lin-Hong Yuan, Lei Zhao, Huan-Ling Yu, Yuan-di Xi, and Rong Xiao.
• School of Public Health, Beijing Key Laboratory of Enviromental Toxicology, Capital Medical University, Beijing 100069, China.
• Afr Health Sci. 2017 Dec 1; 17 (4): 1211-1221.

IntroductionAberrant protein expression within the hippocampus has recently been implicated in the pathogenesis of obesity-induced memory impairment.ObjectivesThe objective of the current study was to search for specific memory-related factors in the hippocampus in obese rats.MethodsSprague-Dawley (SD) rats were fed either a high-fat (HF) diet or normal-fat (NF) diet for 10 weeks to obtain the control (CON), diet-induced obese rats (DIO) and diet-resistant (DR) rats. D-galactose was injected subcutaneously for 10 weeks to establish model (MOD) rats with learning and memory impairment. After the hippocampus of the rats sampling, the proteome analysis was conducted using two-dimensional get electrophoresis (2-DE) combined with peptide mass fingerprinting (PMF).ResultsWe found 15 differential proteins that expressed in the hippocampus in rats induced by HF diet from the 2-DE map. In addition, Neurocalcin-delta (NCALD) was nearly down-regulated in the DR rats compared with CON rats and MOD rats, which was further confirmed by Western blot, real-time PCR and ELISA results.ConclusionOur data demonstrates that the differential memory-related proteins were a reflection of the HF diet, but not potential factors in obesity proneness or obesity resistance. Furthermore, NCALD is proved to be a potential hippocampus-memory related factor related to obesity.

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