• Mojtaba Shahnazi, Majid Zarezadeh Mehrizi, Safar Ali Alizadeh, Peyman Heydarian, Mehrzad Saraei, Mahmood Alipour, and Elham Hajialilo.
• Department of Parasitology, Qazvin University of Medical Sciences, Qazvin, Iran.
• Afr Health Sci. 2019 Mar 1; 19 (1): 1346-1352.

IntroductionHymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method.MethodsA total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite.ResultsOverall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succefully amplified by PCR and further sequenced. The determined sequences were deposited in GenBank under the accession numbers MH337810 -MH337819.ConclusionOur results clarified the presence of H. nana among the patients in the study areas. In addition, the molecular technique could be accessible when the human eggs are the only sources available to identify and diagnose the parasite.

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