• Ahmed Tabbabi, Jabeur Daaboub, Ali Laamari, Raja Ben Cheikh, Mohamed Feriani, Chokri Boubaker, Ibtissem Ben Jha, and Hassen Ben Cheikh.
• Laboratory of Genetics, Faculty of Medicine of Monastir, University of Monastir, Tunisia.
• Afr Health Sci. 2019 Mar 1; 19 (1): 1361-1367.

BackgroundMosquitoes are considered as the main groups of arthropods that cause nuisance and public health problems.ObjectivesEvaluation of resistance to temephos insecticide in Culex pipiens pipiens larvae collected from three districts of Tunisia.MethodsLate third and early fourth instars larvae of Culex pipiens pipiens were collected in three localities of Northern and Southern Tunisia. Field collected populations were tested against temephos insecticide and compared to bioassays of a susceptible reference strain. The cross-resistance between temephos and propoxur, and the polymorphism of over-produced esterases and AChE 1 were investigated.ResultsStudied populations exhibited tolerance to temephos with low and high levels of resistance. The resistance ratio (RR50) values of temephos ranged from 1.34 to 114. Synergists and starch electrophoresis showed that the metabolic resistances were involved in the recorded resistance. Likewise, the resistant target site (acetyl cholinesterase: AChE 1) was responsible for the recorded resistance to temephos compound in Culex pipiens pipiens.ConclusionThe low and high resistance recorded to temephos insecticides is particularly interesting, because it leaves a range of tools useable by vector control services. However, further studies are needed to determine its spread and anticipate vector control failure where these insecticides are used.

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