• Arch Iran Med · Feb 2022

    Effect of Post IORT Wound Fluid Secretion (PIWFS) on the Behavior of Breast Cancer Cells: Stimulator or Inhibitor; Report of an Experimental Study on Breast Cancer.

    • Seyed Mohammadreza Javadi, Mohammad Abdolahad, Solmaz Hashemi, Mohammadali Khayamian, Mohammad Salemizadeh Parizi, Shohreh Vanaei, Hamidreza Mirzaei, Shabnam Jeibouei, Ali Hojat, Hakimeh Zali, Seied Rabi Mahdavi, and Mohammad Esmaeil Akbari.
    • Hamadan University of Medical Sciences, General Surgery Department, Hamadan, Iran.
    • Arch Iran Med. 2022 Feb 1; 25 (2): 78-84.

    BackgroundAlthough investigating the probable side effects of post intraoperative radiotherapy wound fluid secretion (PIWFS) is crucial, especially in clinical cases, no report has been published on the effect of PIWFS on the remaining tumor cells (in the vital state) in cavity side margins or surrounding regions. These tumor cells might be directly/indirectly exposed to intraoperative radiation therapy (IORT). Here, for the first time, we investigated the effect of PIWFS on tumor cells of the same patient extracted from the excised tumor in the spheroid form.MethodsWe generated 8 human-derived breast tumor spheroids from 4 patient specimens who received to IORT, dissociated and cultured them in microfluidic devices. The spheroids from each sample were treated with the patients' PIWFS and DMEM medium separately. Two different parameters, called area and number of detached cells (NDCs), were determined and investigated to evaluate the spheroids' vital and proliferative states.ResultsThe results showed severe transformation in tumor spheroids' function into more invasive and proliferative functions after treatment with PIWFS.ConclusionAlthough the radiation-induced bystander effect may have a role in this observation, further experiments must be done to better clarify the probable desired or non-desired effects of post-IORT secretion for both the remaining tumor cells and the surrounding immune cells.2022 The Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons. org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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