• J Coll Physicians Surg Pak · Sep 2022

    m6A RNA, FTO, ALKBH5 Expression in Type 2 Diabetic and Obesity Patients.

    • Erhan Onalan, Burkay Yakar, Ebru Etem Onalan, Koray Karakulak, Tugce Kaymaz, and Emir Donder.
    • Department of Internal Medicine, Firat University, Medical School, Elazig, Turkey.
    • J Coll Physicians Surg Pak. 2022 Sep 1; 32 (9): 1143-1148.

    ObjectiveTo investigate whether m6A content changes in type 2 diabetes mellitus (T2DM) and obese individuals and whether the relationship of m6A content with the mRNA expression levels of FTO and ALKBH5 genes.Study DesignCross-sectional study.Place And Duration Of StudyDepartment of Internal Medicine, Firat University, Medical School, Elazig, Turkey, between January 2019 and January 2022.MethodologyThe study included 34 newly diagnosed patients with type 2 diabetes mellitus, 34 obese individuals, and 33 healthy individuals without any chronic and metabolic disease matched for age and gender. The global m6A RNA methylation, FTO, and ALKBH5 gene analyses of all the participants were performed. Total cholesterol, triglyceride, LDL, HDL, HbA1c, and insulin and glucose levels were measured.ResultsThe median percentages of m6A RNA methylation in the control group, obese, and T2DM participants were 5.62%, 4.20%, and 5.21% respectively (p=0.004). The m6A RNA methylation percentage of the obese participants was significantly lower than controls (p=0.021). The FTO and ALKBH5 mRNA levels were significantly lower in obese and T2DM participants than in controls. There was a negative significant correlation between m6A RNA level and FTO i.e. (r=-0.291, p=0.003) and ALKBH5 (r=-0.321. p=0.001) levels.Conclusionm6A RNA expression levels of obese individuals were lower than healthy controls. The FTO and ALKBH5 mRNA expressions were lower in both obese and T2DM participants compared to the healthy controls. There was no significant difference between obese and T2DM individuals in terms of m6A RNA expression, FTO and ALKBH5 mRNA expression. m6A RNA expression, FTO, and ALKBH5 levels have a potential role in obesity and diabetes mellitus.Key Wordsm6A RNA methylation, Epigenesis, Genetic, FTO, ALKBH5.

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